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Definitions of services
- C18 ZipTip sample clean up: Desalting, concentration and clean up of samples
prior to MS analysis using Millipore's C18 ZipTips.
- In-gel Trypsin Digestion: Digestion of in-gel protein samples using porcine trypsin.
Price listed below includes sample clean up using ZipTips.
- MS analysis: This will provide accurate Mass measurement for protein or peptides.
MS analysis will include basic searches of trypsin-digested proteins
(Peptide Mass Fingerprinting) against NCBI and/ or SWISS PROT databases.
Unless specified MS analysis will be performed by MALDI-TOF.
- MS/ MS analysis: Tandem MS analysis for the purpose of identification of proteins
by partial sequencing or tagging. This will include an MS analysis and a maximum
(sample dependent) of 5 MS/MS spectra. Unless specified, tandem MS analysis will
be performed on MALDI-QSTAR XL (from Applied Biosystems).
- Nano-LC-MS/MS analysis: Nano-LC MS/MS analysis for the purpose of identification of proteins from a more complex mixture.
In general, analysis is performed using a reverse phase(C18) PepMap column from LC Packings. LC gradients are one hour long.
MS spectra are acquired in IDA mode with each MS cycle consisting of one MS scan (1 sec), followed by 2 MS/MS scans (2 secs/each).
MS/MS is performed on the two most abundant peaks in the MS scan. Resulting data are analyzed either using ProID(Applied Biosystems/MDS Sciex)
or Mascot (Matrix Science). Please contact the proteomics manager for specific requests.
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