Wayne State University (2002, Ph.D.)

Yale University (2004, Postdoctoral Associate)

Assistant Professor, 2004

Associate Professor, 2008

Research in a nut shell

The research in my group focuses in the areas of viral and bacterial infections. Infections caused by either viruses or bacteria involve interactions between a variety of biomolecules such as, DNA, RNA, proteins, metabolites, etc. Tampering with vital bimolecular interactions in an efficient way is the foremost goal of today's medicinal efforts in treating many of the bacterial and viral infections.

In the area of viral infections, specifically in human immunodeficiency virus, we are looking closely at the protein-protein interactions involved during the virus entry to the host cells. With respect to the bacterial infections, we are interested in understanding the induction mechanism of antibiotic resistance factors.

Publications

Thomas V, Golemi-Kotra D., Kim, C., Vakulenko S. B., Mobashery S. and Shoichet B. The effect of S130G mutant in substrate and inhibitor spectrum of TEM-1 β-lactamase. Biochemistry, 2005, 44, 9330-9338.

Golemi-Kotra D., Meroueh S. O., Kim C., Vakulenko S. B. Bulychev A., Stemmer A., Stemmer T. L. and Mobashery S. The importance of a critical protonation state and the fate of the catalytic steps in class A β-lactamases and penicillin binding proteins. J. Biol. Chem. 2004, 279, 34665-73.

Golemi-Kotra D., Mahaffy R., Footer M., Holtzman J., Pollard T., Theriot J. and Schepartz A. High affinity, paralog-specific recognition of the Mena EVH1 domain by a miniature protein ligand. J. Am. Chem. Soc. 2004, 126, 4-5.

Golemi-Kotra D., Young Cha J., Meroueh S. O., Vakulenko S. B. and Mobashery

S. Resistance to β-lactam antibiotics and its mediation by the sensor domain of the transmembrane sensor-transducer signaling pathway in Staphylococcus aureus. J. Biol. Chem. 2003, 278, 18419-18425.

Meroueh S.O., Roblin P., Golemi D., Maveyraud L., Vakulenko S. B. Zhang Y.,

Samama J. P., Mobashery S. Molecular dynamics at the root of expansion of function in the M68L inhibitor-resistant TEM β-lactamase from Escherichia coli. J. Am. Chem. Soc. 2002, 24, 9422-30.

Maveyraud L., Golemi D., Mobashery S., Samama J. P. High-resolution X ray structure of an acyl-enzyme species for the class D OXA-10 β-lactamase. J. Am. Chem. Soc. 2002, 124, 9422-9430.

Vakulenko S. and Golemi D. Mutant TEM β-lactamase produsing resistance to ceftazidime, ampicillins and â-lactamase inhibitors. Antimicrob. Agents Chemother. 2002, 46, 646-653.

Vakulenko S., Golemi D., Geryk B., Souvorov M., Knox J., Mobashery S.,Lerner S. Mutational replacement of Leu-293 in the class C Enterobacter cloacae P99 β-lactamase confers increased MIC of cefepime. Antimicrob. Agents Chemother. 2002, 46, 1966-1970.

Golemi D., Maveyraud L., Vakulenko S., Tranier S., Samama J. P., Mobashery

S. Critical involvement of carbamylated lysine in catalytic function of class D β-lactamases. Proc. Natl. Acad. Sci. USA. 2001, 98, 14280-14285.

Nagase T., Golemi D., Ishiwata A., Mobashery S. Inhibition of β-lactamases by 6,6-bis(hydroxymethyl)penicillanate. Bioorg. Chem. 2001, 29, 140-145.

Golemi-Kotra D. and Mobashery S. Emergence of mechanisms of resistance in

response to the challenge of antibiotics. Encyclopedia on-line, 2002.

Maveyraud L., Golemi D., Vakulenko S., Tranier S., Ishiwata A., Kotra L. P.,

Mobashery S., Samama J. P. Insights into the class D β-lactamases are revealed by the crystal structure of the OXA-10 enzyme in Pseudomonas aeruginosa. Structure. 2000, 8, 1289-1298.

Golemi D., Maveyraud L., Vakulenko S., Tranier S., Ishiwata A., Kotra L. P.,

Samama J. P., Mobashery S. The first structural and mechanistic insights for class D

â-lactamases: Evidence for a novel catalytic process for turnover of β-lactam antibiotics. J. Am. Chem. Soc. 2000, 122, 6132-6133.

Golemi D., Maveyraud L., Ishiwata A., Tranier S., Miyashita K., Nagase T.,

Massova I., Mourey L., Samama J. P., Mobashery S. 6-(xydroxyalkyl)penicillanates as probes for mechanisms of β-lactamases. J. Antibiot. 2000, 53, 1022-1027.

Kotra L. P., Golemi D., Amro N. A., Liu G. Y., Mobashery S. Dynamics of the

lipopolysaccharide assembly on the surface of Escherichia coli. J. Am. Chem.

Soc. 1999, 21, 8707-8711.

Kotra L. P., Golemi D., Amro N. A., Liu G. Y., Mobashery S. Dynamics of the

lipopolysaccharide assembly on the surface of Escherichia coli. J. Am. Chem.

Soc. 1999, 21, 8707-8711.

Swaren P., Golemi D., Cabantous S., Bulychev A., Maveyraud L., Mobashery S.,

Samama J. P. X-ray structure of the Asn276Asp variant of Escherichia coli TEM-1 β-lactamase: Direct observation of electrostatic modulation in resistance to inactivation by clavulanic acid. Biochemistry, 1999, 38, 9570-9576.