List of Publications

Leonard PG, Golemi-Kotra D, Stock AM. Phosphorylation-dependent conformational changes and domain rearrangements in Staphylococcus aureus VraR activation. Proc Natl Acad Sci U S A. 2013 May 21;110(21):8525-30. Epub 2013 May 6.

Rob T, Gill PK, Golemi-Kotra D, Wilson DJ. An electrospray ms-coupled microfluidic device for sub-second hydrogen/deuterium exchange pulse-labelling reveals allosteric effects in enzyme inhibition. Lab Chip. 2013 Feb 21. [Epub ahead of print]

Zhao Y, Verma V, Belcheva A, Singh A, Fridman M, Golemi-Kotra D. Staphylococcus aureus methicillin-resistance factor fmtA is regulated by the global regulator SarA. PLoS One. 2012;7(8):e43998. Epub 2012 Aug 30.

Qamar A, Golemi-Kotra D. Dual Roles of FmtA in Staphylococcus aureus Cell Wall Biosynthesis and Autolysis. Antimicrob Agents Chemother. 2012 Jul;56(7):3797-805. Epub 2012 May 7.

Belcheva A, Verma V, Korenevsky A, Fridman M, Kumar K, Golemi-Kotra D. Roles of DNA sequence and sigma A factor in transcription of the vraSR operon. J Bacteriol. 2012 Jan;194(1):61-71. Epub 2011 Oct 21.

Verma V, Testero SA, Amini K, Wei W, Liu J, Balachandran N, Monoharan T, Stynes S, Kotra LP, Golemi-Kotra D. Hydrolytic mechanism of OXA-58 enzyme, a carbapenem-hydrolyzing class D β-lactamase from Acinetobacter baumannii. J Biol Chem. 2011 Oct 28;286(43):37292-303. Epub 2011 Aug 31.

Liu YH, Belcheva A, Konermann L, Golemi-Kotra D. Phosphorylation-induced activation of the response regulator VraR from Staphylococcus aureus: insights from hydrogen exchange mass spectrometry. J Mol Biol. 2009 Aug 7;391(1):149-63. Epub 2009 Jun 9.

Belcheva A, Verma V, Golemi-Kotra D. DNA-binding activity of the vancomycin resistance associated regulator protein VraR and the role of phosphorylation in transcriptional regulation of the vraSR operon. Biochemistry. 2009 Jun 23;48(24):5592-601.

Belcheva A, Golemi-Kotra D. A close-up view of the VraSR two-component system. A mediator of Staphylococcus aureus response to cell wall damage. J Biol Chem. 2008 May 2;283(18):12354-64. Epub 2008 Mar 7.

Holtzman JH, Woronowicz K, Golemi-Kotra D, Schepartz A. Miniature protein ligands for EVH1 domains: interplay between affinity, specificity, and cell motility. Biochemistry. 2007 Nov 27;46(47):13541-53. Epub 2007 Nov 1.

Fan X, Liu Y, Smith D, Konermann L, Siu KW, Golemi-Kotra D. Diversity of penicillin-binding proteins. Resistance factor FmtA of Staphylococcus aureus. J Biol Chem. 2007 Nov 30;282(48):35143-52. Epub 2007 Oct 9.

Thomas VL, Golemi-Kotra D, Kim C, Vakulenko SB, Mobashery S, Shoichet BK. Structural consequences of the inhibitor-resistant Ser130Gly substitution in TEM beta-lactamase. Biochemistry. 2005 Jul 5;44(26):9330-8.

Golemi-Kotra D, Meroueh SO, Kim C, Vakulenko SB, Bulychev A, Stemmler AJ, Stemmler TL, Mobashery S. The importance of a critical protonation state and the fate of the catalytic steps in class A beta-lactamases and penicillin-binding proteins. J Biol Chem. 2004 Aug 13;279(33):34665-73. Epub 2004 May 19.

Golemi-Kotra D, Mahaffy R, Footer MJ, Holtzman JH, Pollard TD, Theriot JA, Schepartz A. High affinity, paralog-specific recognition of the Mena EVH1 domain by a miniature protein. J Am Chem Soc. 2004 Jan 14;126(1):4-5.

Golemi-Kotra D, Cha JY, Meroueh SO, Vakulenko SB, Mobashery S. Resistance to beta-lactam antibiotics and its mediation by the sensor domain of the transmembrane BlaR signaling pathway in Staphylococcus aureus. J Biol Chem. 2003 May 16;278(20):18419-25. Epub 2003 Feb 18.

Maveyraud L, Golemi-Kotra D, Ishiwata A, Meroueh O, Mobashery S, Samama JP. High-resolution X-ray structure of an acyl-enzyme species for the class D OXA-10 beta-lactamase. J Am Chem Soc. 2002 Mar 20;124(11):2461-5.

Meroueh SO, Roblin P, Golemi D, Maveyraud L, Vakulenko SB, Zhang Y, Samama JP, Mobashery S. Molecular dynamics at the root of expansion of function in the M69L inhibitor-resistant TEM beta-lactamase from Escherichia coli. J Am Chem Soc. 2002 Aug 14;124(32):9422-30.

Vakulenko SB, Golemi D, Geryk B, Suvorov M, Knox JR, Mobashery S, Lerner SA. Mutational replacement of Leu-293 in the class C Enterobacter cloacae P99 beta-lactamase confers increased MIC of cefepime. Antimicrob Agents Chemother. 2002 Jun;46(6):1966-70.

Vakulenko S, Golemi D. Mutant TEM beta-lactamase producing resistance to ceftazidime, ampicillins, and beta-lactamase inhibitors. Antimicrob Agents Chemother. 2002 Mar;46(3):646-53.

Golemi D, Maveyraud L, Vakulenko S, Samama JP, Mobashery S. Critical involvement of a carbamylated lysine in catalytic function of class D beta-lactamases. Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14280-5. Epub 2001 Nov 27.

Nagase T, Golemi D, Ishiwata A, Mobashery S. Inhibition of beta-lactamases by 6,6-bis(hydroxylmethyl)penicillanate. Bioorg Chem. 2001 Jun;29(3):140-5.

Golemi-Kotra D. and Mobashery S. Emergence of mechanisms of resistance in response to the challenge of antibiotics. Encyclopedia on-line, 2002.

Maveyraud L, Golemi D, Kotra LP, Tranier S, Vakulenko S, Mobashery S, Samama JP. Insights into class D beta-lactamases are revealed by the crystal structure of the OXA10 enzyme from Pseudomonas aeruginosa. Structure. 2000 Dec 15;8(12):1289-98.

Golemi D., Maveyraud L., Vakulenko S., Tranier S., Ishiwata A., Kotra LP., Samama JP., Mobashery S. The first structural and mechanistic insights for class D â-lactamases: Evidence for a novel catalytic process for turnover of β-lactam antibiotics. J. Am. Chem. Soc. 2000; 122(23): 6132-6133

Golemi D, Maveyraud L, Ishiwata A, Tranier S, Miyashita K, Nagase T, Massova I, Mourey L, Samama JP, Mobashery S. 6-(hydroxyalkyl)penicillanates as probes for mechanisms of beta-lactamases. J Antibiot (Tokyo). 2000;53(10):1022-7.

Kotra L. P., Golemi D., Amro N. A., Liu G. Y., Mobashery S. Dynamics of the lipopolysaccharide assembly on the surface of Escherichia coli. J. Am. Chem. Soc. 1999; 121(38):8707–8711

Swarén P, Golemi D, Cabantous S, Bulychev A, Maveyraud L, Mobashery S, Samama JP. X-ray structure of the Asn276Asp variant of the Escherichia coli TEM-1 beta-lactamase: direct observation of electrostatic modulation in resistance to inactivation by clavulanic acid. Biochemistry. 1999; 38(30):9570-6.